Comparison of Seed Potato Produced from Different Rapid Multiplication Sources Againts PVX, PVY, And PVS Throug Eliza

Muhammad Burhan, Muhammad Aslam Khan, Muhammad Irfanullah, Muhammad Ishfaq and Muhammad Ehsan Javed

Abstract
These studies were conducted at Ayub Agricultural Research Institute, Faisalabad during 2004. In these studies, 216 leaf samples of three potato varieties (Cardinal, Diamant and Desiree) were compared to know the status of material being produced by rapid multiplication sources through enzyme linked immunosorbent assay (ELISA) against potato virus X (PVX), potato virus Y (PVY) and potato virus S (PVS). The samples were collected from three sources i.e. Punjab Seed Corporation (PSC), Ayub Agricultural Research Institute (AARI) and National Institute of Biotechnology and Genetic Engineering (NIBGE). Out of 72 samples of each variety from three sources, two samples of Cardinal, three samples of Diamant and four samples of Desiree were infected with PVX. Maximum PVX recovery (2.88%) was found in PSC samples as compared to NIBGE (2.16%) and AARI (1.44%). Three samples of each Cardinal and Diamant and five samples of Desiree were infected with PVY. Maximum PVY was found in PSC samples (5.04%) followed by NIBGE (2.16%) and AARI (0.72%). Three samples of Cardinal, four of Diamant and seven samples of Desiree were infected with PVS from three sources. Maximum PVS recovery (5.46%) was found in PSC samples followed by NIBGE (2.88%) and AARI (1.44%) samples.

Keywords: Solanum tuberosum; cultivars; viruses; testing; Pakistan.

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